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  • Indole Test Protocol - American Society for Microbiology
    A positive indole test is indicated by the formation of a pink to red color ("cherry-red ring") in the reagent layer on top of the medium within seconds of adding the reagent (Fig 1b) If a culture is indole negative, the reagent layer will remain yellow or be slightly cloudy (Fig 1c)
  • Indole Test- Principle, Reagents, Procedure, Result Interpretation and . . .
    Quality Control for Indole Test Positive Control: Escherichia coli NCTC 10418 Negative Control: Proteus mirabilis NCTC 10975 Limitations of Indole Test Indole tests may be used as an aid in the identification and differentiation of gram-positive and gram-negative organisms
  • Indole Broth - Thermo Fisher Scientific
    Remel Indole Broth is a liquid medium recommended for use in qualitative procedures to determine indole production by members of the Enterobacteriaceae Indole Broth contains casein peptone, which is rich in the amino acid, tryptophan
  • Indole Test: Principle, Procedure, Results • Microbe Online
    If the rapid indole test is negative, perform a tube test as the isolate could be positive in the more sensitive tube test For fastidious Gram-negative rods, such as C hominis, a heavy inoculum and extraction are necessary
  • Product Detail - Oxoid
    EC Broth is a selective medium for the differentiation of faecal coliforms and the confirmatory test for Escherichia coli from food and environmental samples 3,4 It is also suitable for the enumeration of presumptive E coli in milk and milk products using the most probable number technique 2
  • EC Medium Broth 356-4764 Escherichia coli Broth) - Bio-Rad
    After the incubation period, consider as positive each tube that has given rise to any visible gas in the tube of EC broth and to indole production in the tube of peptone water With the aid of MPN tables, express results according to the specific standard
  • Indole Test- Principle, Media, Procedure, Types, Results, Uses
    If the rapid indole test is negative, the isolate could be positive in the more sensitive tube test Extraction with xylene is the most sensitive test Xylene substitutes are less sensitive
  • 1. SCOPE AND APPLICATION 2. REAGENTS AND MATERIALS REQUIRED - GMP SOP
    negative If at least 2 of the tubes show gas production, proceed with the remainder of the test 3 8 Using a sterile inoculating loop, subculture all cultures as they become positive at 24 hours and 48 hours into EC broth previously warmed to approximately 37 C; include both reference cultures
  • Lauryl Tryptose Mannitol Broth w Tryptophan
    E coli is confirmed by gas and indole production when incubated at 44°C for 24 hours If the indole test is negative even if in a single tube medium, repeat the test in Tryptone Water (M463) Each tube showing acid and gas in the multiple tube test is subcultured to a tube of Lauryl Tryptose Mannitol Broth with Tryptophan and incubated at 44°C
  • Indole test : Principle, Steps, Results - Microbiologie clinique
    The main requirement for a suitable indole test medium is that it contains a sufficient amount of tryptophan (Tryptone broth, indole-free peptone water medium, Urea-indole medium, Tryptophan peptone broth, Sulfide-indole motility medium ( SIM) )





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