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  • Digital PCR in Virology: Current Applications and Future . . . - Springer
    Digital PCR is gaining ground in quantifying low levels of (environmental) DNA, target linkage intactness and mutation detection New developments such as higher-order multiplexing, real-time digital PCR and digital high-resolution melting provide new opportunities within the field of virology
  • Present and Future Applications of Digital PCR in Infectious . . . - MDPI
    Digital PCR, or 3rd generation PCR, is based on a series of technical modifications that result in more sensitive techniques, more resistant to the action of inhibitors and capable of direct quantification without the need for standard curves
  • One-Step RT-qPCR for Viral RNA Detection Using Digital Analysis
    In this study, a simple and rapid thermolysis method was developed to circumvent the need for extraction and purification of viral RNA The developed protocol was applied to one-chip digital PCR (OCdPCR), which allowed thermolysis, RT, and digital PCR in a single unit comprising 20,000 chambers of sub-nanoliter volume
  • Application of Digital PCR as a Reference Measurement Procedure to . . .
    Digital PCR (dPCR) offers a range of potential advantages for nucleic acid analysis, one of which is the accurate quantification of nucleic acid molecules per unit volume
  • Digital PCR Applications in the SARS-CoV-2 COVID-19 Era: a Roadmap for . . .
    In this review article, we selected publications to show the broad-spectrum applications of dPCR, including the development of assays and reference standards, environmental monitoring, mutation detection, and clinical diagnosis of SARS-CoV-2, while comparing it analytically to the gold standard, RT-qPCR
  • Detection and quantification of SARS-CoV-2 by droplet digital PCR in . . .
    Here we evaluated the performance of a ddPCR-based assay to quantify SARS-CoV-2 titer in 55 suspected COVID-19 cases with negative rtPCR results thanks to in-house ddPCR assay (targeting RdRp and host RNaseP) Samples were collected at ASST-GOM Niguarda between February and May 2020 at hospital admission
  • Multiplex detection of respiratory RNA viruses without amplification . . .
    Acute respiratory infections, caused by RNA viruses like respiratory syncytial virus, influenza, rhinovirus, and coronavirus, are major global health threats Real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) is the gold standard for detecting these viruses but is time-consuming, complex, and requires specialized equipment There is a need for rapid, convenient, and multi-target detection methods to improve disease prevention and control This study developed a
  • Microwell Array-based Digital PCR for Influenza Virus Detection
    Digital polymerase chain reaction (dPCR) enables the accurate determination of deoxyribonucleic acid (DNA) copy numbers In contrast to real-time PCR (qPCR), dPCR can perform quantification without requiring previous standard curve experiments Furthermore, dPCR is more sensitive for rare targets in genetic samples
  • Droplet Digital PCR: A Powerful Tool for Accurate Quantification of . . .
    In this work, we develop, validate, and extensively character-ise a one- step reverse transcriptase digital PCR (RT- dPCR)- based measurement method We perform a quantitative and qualitative comparison of RT- dPCR and RT- qPCR of HDV clinical samples with different levels of HDV viremia
  • Application of Digital PCR (dPCR) in Plant Virus Detection
    Abstract Pathogens present a significant risk to both food safety and public health Traditional pathogen detection methods are increasingly inadequate for meeting the demands of modern diagnostics and control Digital PCR (dPCR) has gained substantial … more





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